Polymerase incorporation of pyrene-nucleoside triphosphates

被引：20
|
作者：
Hollenstein, Marcel [1 ]
Wojciechowski, Filip [1 ]
Leumann, Christian J. [1 ]
机构：
[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 13期
基金：
瑞士国家科学基金会;
关键词：
Nucleosides; DNA; Terminal deoxynucleotidyl; transferase (TdT); Nucleotides; Triphosphate analogues; ENZYMATIC-SYNTHESIS; DNA ENZYME; RNA; DNAZYME; ELECTROCHEMISTRY; CLEAVAGE; ANALOG;
D O I：
10.1016/j.bmcl.2012.04.101
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli DNA polymerase I and the TdT polymerase was assessed. The dPTPs are all equally well tolerated by the Klenow fragment, and lead to elongation of up to 5 extra nucleotides of a ssDNA primer in a TdT-mediated reaction. The tailing efficiency of the dPTPs compares favorably to other less drastically modified dNTPs. (C) 2012 Elsevier Ltd. All rights reserved.
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页码：4428 / 4430
页数：3
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