Rapid continuous colorimetric enzyme assay for penicillin G acylase

被引：7
|
作者：
Simons, H [1 ]
Gibson, TD [1 ]
机构：
[1] Appl Enzyme Technol Ltd, Leeds LS2 3AR, W Yorkshire, England
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 06期
关键词：
colorimetric assay; penicillin G acylase; Phenol Red;
D O I：
10.1023/A:1008975600792
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid, continuous, colorimetric enzyme assay for penicillin G acylase has been developed. The assay measures the formation of the acidic products of penicillin G hydrolysis by following the decrease in pH using Phenol Red as an indicator. The activity measured is directly proportional to the amount of enzyme added to the assay, having a linear relationship with an R-2 value of 0.9994.
引用
收藏
页码：365 / 367
页数：3
相关论文
共 50 条
  • [1] Fluorogenic assay for penicillin G acylase activity
    Ninkovic, M
    Riester, D
    Wirsching, F
    Dietrich, R
    Schwienhorst, A
    [J]. ANALYTICAL BIOCHEMISTRY, 2001, 292 (02) : 228 - 233
  • [2] Effect of enzyme inactivation on kinetic parameters of immobilized penicillin G acylase in the hydrolysis of penicillin G
    Holtheuer, J.
    Romero, O.
    Valencia, P.
    Illanes, A.
    Wilson, L.
    [J]. NEW BIOTECHNOLOGY, 2009, 25 : S139 - S139
  • [3] Hollow silica nanotubes for immobilization of penicillin G acylase enzyme
    Xiao, Qing-Gui
    Tao, Xia
    Zhang, Jie-Ping
    Chen, Jian-Feng
    [J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2006, 42 (1-2) : 14 - 19
  • [4] Continuous penicillin G hydrolysis in an electro-membrane reactor with immobilized penicillin G acylase
    Zadrazil, A
    Chmelíková, R
    Hasal, P
    [J]. BIOTECHNOLOGY LETTERS, 2003, 25 (06) : 485 - 490
  • [5] Continuous penicillin G hydrolysis in an electro-membrane reactor with immobilized penicillin G acylase
    Aleš Zadražil
    Růžena Chmelíková
    Pavel Hasal
    [J]. Biotechnology Letters, 2003, 25 : 485 - 490
  • [6] Kinetic model discrimination of penicillin G acylase thermal deactivation by non-isothermal continuous activity assay
    McDonald, Matthew A.
    Bromig, Lukas
    Grover, Martha A.
    Rousseau, Ronald W.
    Bommarius, Andreas S.
    [J]. CHEMICAL ENGINEERING SCIENCE, 2018, 187 : 79 - 86
  • [7] ASSAY OF PENICILLIN ACYLASE IN ORGANIC MEDIA
    ALVES, JRS
    FONSECA, LP
    RAMALHO, MT
    CABRAL, JMS
    [J]. BIOTECHNOLOGY TECHNIQUES, 1995, 9 (10) : 729 - 730
  • [8] Electrically immobilized enzyme reactors: Bioconversion of a charged substrate. Hydrolysis of penicillin G by penicillin G acylase
    Bossi, A
    Guerrera, S
    Righetti, PG
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 1999, 64 (04) : 383 - 391
  • [9] GEL-ENTRAPPED PENICILLIN-G ACYLASE OPTIMIZED BY AN ENZYME THERMISTOR
    WELWARDOVA, A
    GEMEINER, P
    MICHALKOVA, E
    WELWARD, L
    JAKUBOVA, A
    [J]. BIOTECHNOLOGY TECHNIQUES, 1993, 7 (11) : 809 - 814
  • [10] Enzyme flow microcalorimetry - a useful tool for screening of immobilized penicillin G acylase
    Vikartovska-Welwardova, A
    Michalkova, E
    Gemeiner, P
    [J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 1998, 73 (01) : 31 - 36
12345