Design and bioanalytical applications of DNA hairpin-based fluorescent probes

被引：40

作者：

Huang, Jin ^{[1
]}

Yang, Xiaohai ^{[1
]}

He, Xiaoxiao ^{[1
]}

Wang, Kemin ^{[1
]}

Liu, Jianbo ^{[1
]}

Shi, Hui ^{[1
]}

Wang, Qing ^{[1
]}

Guo, Qiuping ^{[1
]}

He, Dinggeng ^{[1
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Coll Biol,Key Lab Bionanotechnol & Mol Engn Hunan, Changsha 410082, Hunan, Peoples R China

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2014年 / 53卷

基金：

中国国家自然科学基金;

关键词：

Aptamer; Bioanalytical application; Biosensor; DNA hairpin; DNA hairpin-based fluorescent probe (DHFP); Dye molecule; Fluorescent probe; Molecular beacon (MB); Nanomaterial; Signal transduction; MOLECULAR BEACONS; NUCLEIC-ACIDS; GRAPHENE OXIDE; EXCIMER PROBES; PROTEIN; HYBRIDIZATION;

D O I：

10.1016/j.trac.2013.08.007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DNA hairpin-based fluorescent probes (DHFPs) are widely used in molecular biology, biosensor development, mRNA monitoring in living cells and imaging in vivo. In this review, we focus on the typical designs and the recent bioanalytical applications of DHEPs. We describe the application of different materials with excellent photophysical properties as signal labels to reduce the background and to increase the brightness of the DHFPs. These labels range from dye molecules to nanomaterials. We also discuss the general design of signal-transduction mechanisms. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：11 / 20

页数：10

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