Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain

被引：4
|
作者：
Tahirov, TH
Morii, H
Uedaira, H
Sarai, A
Ogata, K
机构：
[1] KAST, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[2] Yokohama City Univ, Dept Biol Struct, Sch Med, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[3] Natl Inst Biosci & Human Technol, Prot Folding Grp, NIBH, Tsukuba, Ibaraki 3058566, Japan
[4] RIKEN, Inst Phys & Chem Res, Tsukuba Life Sci Ctr, Tsukuba, Ibaraki 3050074, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷
关键词：
D O I：
10.1107/S0907444999005041
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The R2 subdomain of the mouse c-Myb DNA-binding domain and its V103L mutant have been crystallized by the vapour-diffusion method using highly concentrated sodium citrate at pH 6.8 as a precipitant. Using ammonium sulfate as precipitant in MES buffer only produced crystals for the mutant R2. All crystals are isomorphous and belong to space group P2(1)2(1)2(1). The unit-cell dimensions for wild-type R2 crystals grown from sodium citrate precipitant are a = 28.83, b = 40.18, c = 49.23 Angstrom. Crystals contain one R2 molecule per asymmetric unit. They are stable during 3 d exposure to X-rays and diffract to 1.37-1.45 Angstrom resolution.
引用
收藏
页码：1345 / 1347
页数：3
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