Spectrophotometric Determination of Quinolone Antibiotics by an Association Complex Formation with Aluminum(III) and Erythrosin

被引:9
|
作者
Yamaguchi, Takako [1 ]
Nakao, Masahiro [1 ]
Nakahara, Ryosuke [1 ]
Njishioka, Yuka [1 ]
Ikeia, Chiho [1 ]
Fujia, Yoshikazu [1 ]
机构
[1] Osaka Univ Pharmaceut Sci, Osaka 5691094, Japan
关键词
PERFORMANCE LIQUID-CHROMATOGRAPHY; OFLOXACIN; DNA; PHARMACEUTICALS; HYDROCHLORIDE; DERIVATIVES; EQUILIBRIA; PROTEIN; GYRASE; ION;
D O I
10.2116/analsci.25.125
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple and sensitive spectrophotometric method for the determination of quinolone antibiotics was established based on an association complex formation with aluminum(III) and erythrosin. In the determination of ofloxacin as a quinolone antibiotic, Beer's law is obeyed in the range of 0.1 - 3.2 mu g ml(-1), with an effective molar absorptivity at 555 nm and the relative standard deviation being 1.2 x 10(5) L mol(-1) cm(-1) and 0.9% (n = 6). This method was Successfully applied to the assay of quinolone antibiotics in pharmaceutical preparations.
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页码:125 / 128
页数:4
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