Isolation of tumor cells from peripheral blood - comparison of PCR and immunochemistry

被引：0
|
作者：
Illert, B [1 ]
Horak, KH [1 ]
Timmermann, W [1 ]
Otto, C [1 ]
Thiede, A [1 ]
机构：
[1] Univ Wurzburg, Dept Surg, D-97070 Wurzburg, Germany
来源：
2ND INTERNATIONAL CONGRESS ON GASTROENTEROLOGICAL CARCINOGENESIS | 1999年
关键词：
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Detection of tumor cells is discussed as a prognostic marker in malignant disease. Two different methods for detection of tumor cells from peripheral venous blood were compared using the polymerase-chain-reaction and immunochemistry. With both methods tumor cells can be detected in concentrations up to 10 cells/five mi of blood. Combination of both methods facilitates high sensitivity and visual proof of tumor cells in vitro. Attempt, method and benefit of this approach must be proofed concerning a definite clinical use.
引用
收藏
页码：195 / 197
页数：3
相关论文
共 50 条
  • [1] ISOLATION OF TUMOR CELLS FROM BLOOD - APPRAOCHES TO TECHNIQUE COMPARISON
    WATNE, AL
    ACTA CYTOLOGICA, 1965, 9 (02) : 123 - &
  • [2] Isolation of RNA from equine peripheral blood cells: comparison of methods
    Jiang, Zibin
    Uboh, Cornelius E.
    Chen, Jinwen
    Soma, Lawrence R.
    SPRINGERPLUS, 2013, 2 : 1 - 6
  • [3] Isolation and expansion the circulating tumor cells from patients' peripheral blood in vitro
    Zhou, W.
    Zou, C.
    Zhao, P.
    Liu, C.
    Zhang, H.
    Hu, H.
    Zhong, C.
    Zhang, Y.
    Zhou, D.
    CANCER RESEARCH, 2019, 79 (04)
  • [4] Isolation of human myeloid dendritic cells from tumor tissue and peripheral blood
    Brocks, C
    Graefe, H
    Frenzel, H
    Pries, R
    Wollenberg, B
    IN VIVO, 2006, 20 (02): : 239 - 242
  • [5] Genomic PCR detects tumor cells in peripheral blood from patients with myxoid liposarcoma
    Panagopoulos, I
    Aman, P
    Mertens, F
    Mandahl, N
    Rydholm, A
    Bauer, HFC
    Mitelman, F
    GENES CHROMOSOMES & CANCER, 1996, 17 (02): : 102 - 107
  • [6] Isolation of endothelial cells and their progenitor cells from human peripheral blood
    Boyer, M
    Townsend, LE
    Vogel, LM
    Falk, J
    Reitz-Vick, D
    Trevor, KT
    Villalba, M
    Bendick, PJ
    Glover, JL
    JOURNAL OF VASCULAR SURGERY, 2000, 31 (01) : 181 - 189
  • [7] FABRICATION AND TESTING OF A MAGNETOPHORETIC BIOSEPARATION CHIP FOR ISOLATION AND DETECTION OF CIRCULATING TUMOR CELLS FROM PERIPHERAL BLOOD
    Jackson, Seth
    Darabi, Jeff
    Schober, Joseph
    PROCEEDINGS OF THE ASME/JSME/KSME JOINT FLUIDS ENGINEERING CONFERENCE, 2019, VOL 4, 2019,
  • [8] Isolation of Circulating Tumor Cells from Peripheral Blood Samples of Cancer Patients Using Microfluidic Technology
    Volovetskiy, A. B.
    Malinina, P. A.
    Kapitannikova, A. Y.
    Smetanina, S., V
    Kruglova, I. A.
    Maslennikova, A., V
    SOVREMENNYE TEHNOLOGII V MEDICINE, 2020, 12 (06) : 62 - 69
  • [9] EFFICIENT ISOLATION OF TROPHOBLAST CELLS FROM PERIPHERAL-BLOOD
    DURRANT, LG
    MCDOWALL, KM
    LIU, DTY
    FETAL CELLS IN MATERNAL BLOOD: PROSPECTS FOR NONINVASIVE PRENATAL DIAGNOSIS, 1994, 731 : 175 - 177
  • [10] Methods for isolation of hemopoiesis precursor cells from peripheral blood
    Ptushkin, VV
    GEMATOLOGIYA I TRANSFUZIOLOGIYA, 2000, 45 (06): : 43 - 45
12345