Antifungal resistance of Candida glabrata vaginal isolates and development of a quantitative reverse transcription-PCR-based azole susceptibility assay

被引：17
|
作者：
Gygax, Scott E. [1 ]
Vermitsky, John-Paul
Chadwick, Sean G.
Self, Matthew J.
Zimmerman, Jessica A.
Mordechai, Eli
Adelson, Martin E.
Trama, Jason P. [2 ]
机构：
[1] Med Diagnost Labs LLC, Dept Res & Dev, Antimicrobial Resistance Div, Hamilton, NJ 08690 USA
[2] Mol & Cellular Biol Div, Hamilton, NJ 08690 USA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2008年 / 52卷 / 09期
关键词：
D O I：
10.1128/AAC.00462-08
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A multiplex quantitative reverse transcription-PCR assay was developed to detect azole resistance in Candida glabrata, an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a >= 3-fold increase in CDR1 expression by this assay, which proved to be 100% sensitive and 95% specific in comparison to the gold standard broth microdilution assay.
引用
收藏
页码：3424 / 3426
页数：3
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