A photocleavable surfactant for top-down proteomics

被引：82

作者：

Brown, Kyle A. ^{[1
]}

Chen, Bifan ^{[1
]}

Guardado-Alvarez, Tania M. ^{[1
]}

Lin, Ziqing ^{[2
,3
]}

Hwang, Leekyoung ^{[1
]}

Ayaz-Guner, Serife ^{[2
]}

Jin, Song ^{[1
]}

Ge, Ying ^{[1
,2
,3
]}

机构：

[1] Univ Wisconsin, Dept Chem, 1101 Univ Ave, Madison, WI 53706 USA

[2] Univ Wisconsin, Sch Med & Publ Hlth, Dept Cell & Regenerat Biol, Madison, WI 53706 USA

[3] Univ Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA

来源：

NATURE METHODS | 2019年 / 16卷 / 05期

基金：

美国国家卫生研究院;

关键词：

SPECTROMETRY-COMPATIBLE SURFACTANT; MASS-SPECTROMETRY; MEMBRANE-PROTEINS; SOFTWARE TOOL;

D O I：

10.1038/s41592-019-0391-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS-polyacrylamide gel electrophoresis.

引用

页码：417 / +

页数：7

共 50 条

[41] Novel Strategies to Address the Challenges in Top-Down Proteomics
Melby, Jake A.
Roberts, David S.
Larson, Eli J.
Brown, Kyle A.
Bayne, Elizabeth F.
Jin, Song
Ge, Ying
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2021, 32 (06) : 1278 - 1294
[42] Enhancing Top-Down Proteomics of Brain Tissue with FAIMS
Fulcher, James M.
Makaju, Aman
Moore, Ronald J.
Zhou, Mowei
Bennett, David A.
De Jager, Philip L.
Qian, Wei-Jun
Pasa-Tolic, Ljiljana
Petyuk, Vladislav A.
JOURNAL OF PROTEOME RESEARCH, 2021, 20 (05) : 2780 - 2795
[43] Top-down microbial proteomics for phenotypic biomarker discovery
不详
MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (08) : S487 - S487
[44] Optimized Proteome Reduction for Integrative Top-Down Proteomics
Woodland, Breyer
Necakov, Aleksandar
Coorssen, Jens R. R.
PROTEOMES, 2023, 11 (01)
[45] Top-Down Garbage Collector: a tool for selecting high-quality top-down proteomics mass spectra
Lima, Diogo B.
Silva, Andre R. F.
Dupre, Mathieu
Santos, Marlon D. M.
Clasen, Milan A.
Kurt, Louise U.
Aquino, Priscila F.
Barbosa, Valmir C.
Carvalho, Paulo C.
Chamot-Rooke, Julia
BIOINFORMATICS, 2019, 35 (18) : 3489 - 3490
[46] Top-down characterization of endogenous protein complexes with native proteomics
Skinner, Owen S.
Haverland, Nicole A.
Fornelli, Luca
Melani, Rafael D.
Do Vale, Luis H. F.
Seckler, Henrique S.
Doubleday, Peter F.
Schachner, Luis F.
Srzentic, Kristina
Kelleher, Neil L.
Compton, Philip D.
NATURE CHEMICAL BIOLOGY, 2018, 14 (01) : 36 - +
[47] Proteomics in heart failure: top-down or bottom-up?
Zachery R. Gregorich
Ying-Hua Chang
Ying Ge
Pflügers Archiv - European Journal of Physiology, 2014, 466 : 1199 - 1209
[48] Spatial top-down proteomics for the functional characterization of human kidney
Zemaitis, Kevin J.
Fulcher, James M.
Kumar, Rashmi
Degnan, David J.
Lewis, Logan A.
Liao, Yen-Chen
Velickovic, Marija
Williams, Sarah M.
Moore, Ronald J.
Bramer, Lisa M.
Velickovic, Dusan
Zhu, Ying
Zhou, Mowei
Pasa-Tolic, Ljiljana
CLINICAL PROTEOMICS, 2025, 22 (01)
[49] A perspective view of top-down proteomics in snake venom research
Ghezellou, Parviz
Garikapati, Vannuruswamy
Kazemi, Seyed Mahdi
Strupat, Kerstin
Ghassempour, Alireza
Spengler, Bernhard
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2019, 33 : 20 - 27
[50] Mesh Fragmentation Improves Dissociation Efficiency in Top-down Proteomics
Lu, Lei
Scalf, Mark
Shortreed, Michael R.
Smith, Lloyd M.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2021, 32 (06) : 1319 - 1325

← 1 2 3 4 5 →