A photocleavable surfactant for top-down proteomics

被引：82

作者：

Brown, Kyle A. ^{[1
]}

Chen, Bifan ^{[1
]}

Guardado-Alvarez, Tania M. ^{[1
]}

Lin, Ziqing ^{[2
,3
]}

Hwang, Leekyoung ^{[1
]}

Ayaz-Guner, Serife ^{[2
]}

Jin, Song ^{[1
]}

Ge, Ying ^{[1
,2
,3
]}

机构：

[1] Univ Wisconsin, Dept Chem, 1101 Univ Ave, Madison, WI 53706 USA

[2] Univ Wisconsin, Sch Med & Publ Hlth, Dept Cell & Regenerat Biol, Madison, WI 53706 USA

[3] Univ Wisconsin, Sch Med & Publ Hlth, Human Prote Program, Madison, WI 53706 USA

来源：

NATURE METHODS | 2019年 / 16卷 / 05期

基金：

美国国家卫生研究院;

关键词：

SPECTROMETRY-COMPATIBLE SURFACTANT; MASS-SPECTROMETRY; MEMBRANE-PROTEINS; SOFTWARE TOOL;

D O I：

10.1038/s41592-019-0391-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS-polyacrylamide gel electrophoresis.

引用

页码：417 / +

页数：7

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