Nonomuraea nitratireducens sp. nov., a new actinobacterium isolated from Suaeda australis Moq. rhizosphere

A novel actinomycete, designated WYY166(T), was isolated from the rhizosphere of Suaeda australis Moq. collected in Dongfang, PR China. The taxonomic position of this strain was investigated using a polyphasic approach. Phylogenetic analysis based on its 16S rRNA gene referred strain WYY166(T) to the genus Nonomuraea, and it was most closely related to the type strains Nonomuraea candida HMC10(T), Nonomuraea turkmeniaca DSM 43926(T), Nonomuraea maritima NBRC 106687(T) and Nonomuraea polychroma DSM 43925(T) (98.35, 97.60, 97.36 and 97.30% sequence similarity, respectively). Genome sequencing revealed a genome size of 11.27 Mbp and a G+C content of 71.10 mol%. The genome average nucleotide identity (ANI) values and the digital DNA - DNA hybridization (dDDH) values between strain WYY166(T) and the other species of the genus were found to be low (ANI 81.63 similar to 85.23%. dDDH 23.6 similar to 31.6%), suggesting that it represented a new species. The physiological evaluation showed that it had remarkable nitrate reduction activity. The whole-cell hydrolysates contained meso-diaminopimelic acid and madurose. The N-acyl type of muramic acid was acetyl. The major menaquinones were MK-9 (H-4) (86.9%) and MK-9 (H-2) (13.1%). The predominant fatty acids were iso-C-1(6:)0 (53.2%), 10-methyl C-17:0 (10.7%), C-17:1 omega 6c (8.3%) and iso-C(16:1)h (7.3%). These physiological, biochemical and chemotaxonomic data suggested that strain WYY166(T) should be classified as representing a novel species of the genus Nonomuraea, for which the name Nonomuraea nitratireducens sp. nov. is proposed. The type strain is WYY166(T) (=MCCC 1K03779(T)=KCTC 49343(T)).