Intratumoral low-volume jet-injection for efficient nonviral gene transfer

被引：38

作者：

Walther, W ^{[1
]}

Stein, U ^{[1
]}

Fichtner, I ^{[1
]}

Voss, C ^{[1
]}

Schmidt, T ^{[1
]}

Schleef, M ^{[1
]}

Nellessen, T ^{[1
]}

Schlag, PM ^{[1
]}

机构：

[1] Max Delbruck Ctr Mol Med, D-13092 Berlin, Germany

来源：

MOLECULAR BIOTECHNOLOGY | 2002年 / 21卷 / 02期

关键词：

gene therapy; nonviral gene transfer; jet-injection; naked DNA; plasmid topology; plasmid stability;

D O I：

10.1385/MB:21:2:105

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Jet-injection has become an applicable technology among other established nonviral delivery systems, such as particle bombardment or in vivo electroporation. The low-volume jet injector employed in this study uses compressed air to inject solutions of 1.5-10 muL containing naked DNA into the desired tissue. The, novel design of this prototype makes multiple jet-injections possible. Therefore, repeated jet-injections into one target tissue can be performed easily. This jet-injector hand-held system was used for the direct in vivo gene transfer of plasmid DNA into tumors to achieve efficient expression of reporter genes (beta-galactosidase, green fluorescent protein [GFP]) and of therapeutic genes (TNF-alpha) in different tumor models. The study presented here revealed the key parameters of efficient in vivo jet-injection (jet-injection volume, pressure, jet penetration, DNA stability) to define the optimal conditions for a jet-injection-aided nonviral gene therapy.

引用

页码：105 / 115

页数：11

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