Microsatellite markers for the Amazon peacock bass (Cichla piquiti)

被引:7
|
作者
Carvalho, D. C. [1 ,2 ]
Oliveira, D. A. A. [2 ]
Sampaio, I. [3 ]
Beheregaray, L. B. [1 ]
机构
[1] Macquarie Univ, Dept Biol Sci, Mol Ecol Lab, Sydney, NSW 2109, Australia
[2] Univ Fed Minas Gerais, Dept Zootecnia, Lab Genet Anim, Belo Horizonte, MG, Brazil
[3] Fed Univ Para, Inst Estudos Costeiros, Lab Genet & Biol Mol, Braganca, PA, Brazil
关键词
Cichlidae; conservation genetics; introduced populations; microsatellites; Peacock bass; TucunarE;
D O I
10.1111/j.1755-0998.2008.02425.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A set of primers to amplify 10 microsatellite DNA loci was developed for the Neotropical fish Cichla piquiti, one of the largest sized cichlids in the Amazon Basin. These loci were used to genotype individuals from two populations, one native population from the Tocantins River, the other an introduced population in southeast Brazil, Upper Parana River. Cross-amplification was also successful for another species of peacock bass, C. kelberi. An average of 4.4 alleles per locus (2-9 alleles) was detected. These markers will be useful for the characterization of genetic structure of native populations, and also for invasive biology studies since Cichla species have been introduced in many river basins outside their native ranges.
引用
收藏
页码:239 / 241
页数:3
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