Expression profiling of the bottom fermenting yeast Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays

被引:13
|
作者
Minato, Toshiko [1 ]
Yoshida, Satoshi [1 ]
Ishiguro, Tatsuji [1 ]
Shimada, Emiko [1 ]
Mizutani, Satoru [1 ]
Kobayashi, Osamu [1 ]
Yoshimoto, Hiroyuki [1 ]
机构
[1] Kirin Holdings Co Ltd, Cent Labs Frontier Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
关键词
Saccharomyces pastorianus; bottom fermenting yeast; oligonucleotide microarrays; orthologue; LAGER BREWING YEAST; CEREVISIAE; CARLSBERGENSIS; TRANSCRIPTION; FERMENTATION; RNA;
D O I
10.1002/yea.1654
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The bottom fermenting yeast Saccharomyces pastorianus is reported to have arisen as a natural hybrid of two yeast strains, S. cerevisiae and S. bayanus. The S. pastorianus genome includes S. cerevisiae-type (Se-type) genes and orthologous lager-fermenting-yeast specific-type (Lg-type) genes derived from S. cerevisiae and S. bayallus, respectively. To gain insights into the physiological properties of S. pastorianus, we developed an in situ synthesized 60-mer oligonucleotide microarray for gene expression monitoring of these orthologous genes, consisting of approximately 6600 Sc-type genes and 3200 Lg-type genes. A comparison of the transcriptional profile of orthologous genes (e.g. Sc-type and Lg-type genes) in S. cerevisiae or S. bayallus demonstrated the feasibility of performing gene expression studies with this microarray. Genome-wide analysis of S. pastorianus with this microarray could clearly distinguish more than 67% of the expressed orthologous genes. Furthermore, it was shown that the gene expression of particular Lg-type genes differed from that of the orthologous Sc-type genes, suggesting that some Lg-type and Sc-type genes may have different functional roles. We conclude that the oligonucleotide microarray that we constructed is a powerful tool for the monitoring of gene expression of the orthologous genes of S. pastorianus. Copyright (c) 2009 John Wiley & Sons, Ltd.
引用
收藏
页码:147 / 165
页数:19
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