CLIP-170 tethers kinetochores to microtubule plus ends against poleward force by dynein for stable kinetochore-microtubule attachment

被引：5
|
作者：
Amin, Mohammed Abdullahel [1 ]
Kobayashi, Kinue [1 ]
Tanaka, Kozo [1 ]
机构：
[1] Tohoku Univ, Dept Mol Oncol, Inst Dev Aging & Canc, Sendai, Miyagi 9808575, Japan
来源：
FEBS LETTERS | 2015年 / 589卷 / 19期
关键词：
Cytoplasmic linker protein-170; Dynein; Chromosome alignment; Kinetochore; Microtubule; Mitosis; CHROMOSOME ALIGNMENT; MITOSIS; P150(GLUED); BINDING; PLK1; AUTOINHIBITION; CONGRESSION; MECHANISMS; DYNACTIN; TRACKING;
D O I：
10.1016/j.febslet.2015.07.036
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cytoplasmic linker protein (CLIP)-170 localizes to kinetochores and is suggested to function in stable attachment of kinetochores to microtubule ends. Here we show that defects in kinetochore-microtubule attachment and chromosome alignment in CLIP-170-depleted cells were rescued by co-depletion of p150glued, a dynactin subunit required for kinetochore localization of CLIP-170. CLIP-170 recruited p150glued to microtubule ends. Kinetochore localization at microtubule ends was perturbed by CLIP-170 depletion, which was rescued by co-depleting p150glued. Our results imply that CLIP-170 tethers kinetochores to microtubule ends against the dynein-mediated pole-ward force to slide kinetochores along microtubules, facilitating the stable kinetochore attachment to microtubules. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：2739 / 2746
页数：8
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