Protein Peptide Purification using the Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process

被引：0
|
作者：
Aumann, Lars [1 ]
Stroehlein, Guido [1 ]
Mueller-Spaeth, Thomas [1 ]
Schenkel, Berthold [3 ]
Morbidelli, Massimo [2 ]
机构：
[1] ChromaCon AG, Zurich, Switzerland
[2] ETH, Zurich, Switzerland
[3] Novartis Pharma AG, Technol Grp 1, CHBS, Basel, Switzerland
来源：
BIOPHARM INTERNATIONAL | 2009年 / 22卷 / 01期
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D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Increasing upstream yields have led to the need for downstream purification capacities. In this article, a new chromatographic process for the purification of biomolecules is presented. Its working principle is explained and possible areas of application in monoclonal antibody and polypeptide purification are discussed. For an industrial polypeptide purification problem, experimental work showed that a multicolumn countercurrent solvent gradient purification (MCSGP) process could raise the productivity 25-fold, compared to the current batch chromatographic step in production.
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页码：46 / +
页数：5
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