Microbial synthesis of poly-3-hydroxybutyrate and its application as targeted drug delivery vehicle

被引：28
|
作者：
Althuri, Avanthi [1 ,2 ]
Mathew, Jincy [1 ]
Sindhu, Raveendran [1 ]
Banerjee, Rintu [2 ]
Pandey, Ashok [1 ]
Binod, Parameswaran [1 ]
机构：
[1] CSIR, Natl Inst Interdisciplinary Sci & Technol, Div Biotechnol, Trivandrum 695019, Kerala, India
[2] Indian Inst Technol, Agr & Food Engn Dept, Microbial Biotechnol & Downstream Proc Lab, Kharagpur 721302, W Bengal, India
来源：
BIORESOURCE TECHNOLOGY | 2013年 / 145卷
关键词：
Polyhydroxybutyrate; Arsenic trioxide loaded; polyhydroxybutyrate-polyvinyl alcohol; copolymer nanoparticles; Folate conjugated nanoparticles; Folate receptor positive; PLGA NANOPARTICLES; IN-VITRO; CELLS; POLY(3-HYDROXYBUTYRATE); BIODEGRADATION; APOPTOSIS; VARIABLES; BEHAVIOR; CULTURE; ACID);
D O I：
10.1016/j.biortech.2013.01.106
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Arsenic trioxide loaded biocompatible PHB-PVA(1) nanoparticles (<100 nm in size) with folate functionalized surface were synthesized using poly-[(R)-3-hydroxybutyric acid] (PHB) produced by Bacillus firmus NII 0830. Folate functionalization was carried using dicyclohexyl carbodiimide (DCC) as a catalyst and 10-bromodecanol as a linker to conjugate glutamic acid terminal of folate with the hydroxylate groups present on the surface of PHBA-PVA(2) nanotrojans. The effect of fabrication parameters on shape, size distribution and PDI of the PHB nanoparticles were also investigated. It was observed that increase in sonication time and polyvinyl alcohol (PVA) concentration greatly reduced the size of nanoparticles. The drug release studies on arsenic trioxide incorporated PHB-PVA nanoparticles were conducted at physiological pH and temperature. FOL-PHBA-PVA(3) nanoparticles showed greater extent of cytotoxicity towards murine fibrosarcoma L929 cells than PHBA-PVA nanoparticles alone without conjugated folate, indicating the significance of folate as ligand for specific targeting of FR+ cancer cells. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：290 / 296
页数：7
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