Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

被引:8
|
作者
Muentefering, Thomas [1 ]
Michels, Alexander P. E. [1 ]
Pfeuffer, Steffen [1 ]
Meuth, Sven G. [1 ]
Ruck, Tobias [1 ]
机构
[1] Univ Munster, Inst Translat Neurol & Neurol Clin, Munster, Germany
来源
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2019年 / 145期
关键词
Neuroscience; Issue; 145; Cell isolation; murine microvascular endothelial cells; skeletal muscle cells; blood muscle barrier; myovascular unit; myopathy;
D O I
10.3791/58901
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The endothelial cells of skeletal muscle capillaries (muscle microvascular endothelial cells, MMEC) build up the barrier between blood stream and skeletal muscles regulating the exchange of fluids and nutrients as well as the immune response against infectious agents by controlling immune cell migration. For these functions, MMEC form a functional "myovascular unit" (MVU), with further cell types, such as fibroblasts, pericytes and skeletal muscle cells. Consequently, a dysfunction of MMEC and therefore the MVU contributes to a vast variety of myopathies. However, regulatory mechanisms of MMEC in health and disease remain insufficiently understood and their elucidation precedes more specific treatments for myopathies. The isolation and in-depth investigation of primary MMEC functions in the context of the MVU might facilitate a better understanding of these processes. This article provides a protocol to isolate primary murine MMEC of the skeletal muscle by mechanical and enzymatic dissociation including purification and culture maintenance steps.
引用
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页数:8
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