A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

被引：37

作者：

Perry, Sarah L. ^{[1
]}

Guha, Sudipto ^{[1
]}

Pawate, Ashtamurthy S. ^{[1
]}

Bhaskarla, Amrit ^{[2
]}

Agarwal, Vinayak ^{[3
]}

Nair, Satish K. ^{[3
]}

Kenis, Paul J. A. ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL USA

[2] Univ Illinois, Sch Mol & Cellular Biol, Urbana, IL USA

[3] Univ Illinois, Dept Biochem, Urbana, IL USA

来源：

LAB ON A CHIP | 2013年 / 13卷 / 16期

关键词：

CRYSTAL-STRUCTURE; MEMBRANE-PROTEINS; CRYSTALLIZATION; SYSTEM; PURIFICATION; PLATFORM;

D O I：

10.1039/c3lc50276g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 angstrom via on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.

引用

页码：3183 / 3187

页数：5

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