Single-Molecule FRET Reveals the Folding Dynamics of the Human Telomerase RNA Pseudoknot Domain

被引：48
|
作者：
Hengesbach, Martin [1 ]
Kim, Nak-Kyoon [3 ]
Feigon, Juli [2 ]
Stone, Michael D. [1 ]
机构：
[1] Univ Calif Santa Cruz, Ctr Mol Biol RNA, Dept Chem & Biochem, Santa Cruz, CA 95064 USA
[2] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
[3] Korea Inst Sci & Technol, Adv Anal Ctr, Seoul, South Korea
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 24期
关键词：
FRET; NMR spectroscopy; RNA structures; single-molecule studies; DYSKERATOSIS-CONGENITA; TRIPLE-HELIX; IN-VITRO;
D O I：
10.1002/anie.201200526
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Do knot FRET: Single-molecule FRET was used to analyze the folding of human telomerase RNA (see scheme) and allowed discrimination between the correctly folded(left) and misfolded(right) structures. Analyzing the effects of various mutations showed that base pairing, triplex formation, and Mg 2+ ion binding act synergistically in the folding of the pseudoknot structure in a functional hTR construct. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：5876 / 5879
页数：4
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