Serum soluble Fas ligand is a severity and mortality prognostic marker for COVID-19 patients

被引:14
|
作者
Saleki, Kiarash [1 ,2 ,3 ,4 ]
Shirzad, Moein [1 ,2 ,4 ]
Javanian, Mostafa [5 ]
Mohammadkhani, Sheyda [2 ]
Alijani, Mohammad Hossein [2 ]
Miri, Niloufarsadat [1 ]
Oladnabi, Morteza [6 ]
Azadmehr, Abbas [1 ,4 ,7 ]
机构
[1] Babol Univ Med Sci, Hlth Res Inst, Cellular & Mol Biol Res Ctr, Babol, Iran
[2] Babol Univ Med Sci, Student Res Comm, Babol, Iran
[3] Babol Univ Med Sci, USERN Off, Babol, Iran
[4] Natl Elite Fdn, Mazandaran Prov Branch, Mazandaran, Iran
[5] Babol Univ Med Sci, Hlth Res Inst, Infect Dis & Trop Med Res Ctr, Babol, Iran
[6] Golestan Univ Med Sci, Ischem Disorders Res Ctr, Gorgan, Iran
[7] Babol Univ Med Sci, Immunol Dept, Babol, Iran
来源
FRONTIERS IN IMMUNOLOGY | 2022年 / 13卷
关键词
COVID-19; hyperinflammation; Fas; viral Immunology; immunoinformatics; AA FORCE-FIELD; EXPRESSION; APOPTOSIS; INJURY; ACTIVATION; VIRUS; GENE;
D O I
10.3389/fimmu.2022.947401
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Finding cytokine storm initiator factors associated with uncontrolled inflammatory immune response is necessary in COVID-19 patients. The aim was the identification of Fas/Fas Ligand (FasL) role in lung involvement and mortality of COVID-19 patients. In this case-control study, mild (outpatient), moderate (hospitalized), and severe (ICU) COVID-19 patients and healthy subjects were investigated. RNA isolated from PBMCs for cDNA synthesis and expression of mFas/mFasL mRNA was evaluated by RT-PCR. Serum sFas/sFasL protein by ELISA and severity of lung involvement by CT-scan were evaluated. Also, we docked Fas and FasL via Bioinformatics software (in silico) to predict the best-fit Fas/FasL complex and performed molecular dynamics simulation (MDS) in hyponatremia and fever (COVID-19 patients), and healthy conditions. mFasL expression was increased in moderate and severe COVID-19 patients compared to the control group. Moreover, mFas expression showed an inverse correlation with myalgia symptom in COVID-19 patients. Elevation of sFasL protein in serum was associated with reduced lung injury and mortality. Bioinformatics analysis confirmed that blood profile alterations of COVID-19 patients, such as fever and hyponatremia could affect Fas/FasL complex interactions. Our translational findings showed that decreased sFasL is associated with lung involvement; severity and mortality in COVID-19 patients. We think that sFasL is a mediator of neutrophilia and lymphopenia in COVID-19. However, additional investigation is suggested. This is the first report describing that the serum sFasL protein is a severity and mortality prognostic marker for the clinical management of COVID-19 patients.
引用
收藏
页数:20
相关论文
共 50 条
  • [21] Serum levels of soluble Fas ligand in patients with silicosis
    Tomokuni, A
    Otsuki, T
    Isozaki, Y
    Kita, S
    Ueki, H
    Kusaka, M
    Kishimoto, T
    Ueki, A
    CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1999, 118 (03): : 441 - 444
  • [22] SERUM SOLUBLE FAS AND SOLUBLE FAS LIGAND IN HYPERTENSION IN PREGNANCY
    Karthikeyan, Vellore J.
    Jessani, Shahirose
    Balakrishnan, Balu
    Lane, Deirdre A.
    Baghdadi, Sabah
    Beevers, D. Gareth
    Lip, Gregory Y. H.
    JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2010, 55 (10)
  • [23] Homocysteine as a marker for predicting disease severity in patients with COVID-19
    Keskin, Adem
    Ustun, Goksenin U.
    Aci, Recai
    Duran, Utku
    BIOMARKERS IN MEDICINE, 2022, 16 (07) : 559 - 568
  • [24] Ferritin as a Marker of Severity in COVID-19 Patients: A Fatal Correlation
    Dahan, Shani
    Segal, Gad
    Katz, Itai
    Hellou, Tamar
    Tietel, Michal
    Bryk, Gabriel
    Amital, Howard
    Shoenfeld, Yehuda
    Dagan, Amir
    ISRAEL MEDICAL ASSOCIATION JOURNAL, 2020, 22 (08): : 428 - 434
  • [25] Systemic immune-inflammation index (SII) as a prognostic marker of mortality in COVID-19 patients
    Sandoval-Bedolla, Karen L.
    Elizalde-Barrera, Cesar I.
    Garcia-Lopez, Victor H.
    Huerta-Ramirez, Saul
    Martinez-Cardozo, Daniel
    CIRUGIA Y CIRUJANOS, 2023, 91 (03): : 361 - 367
  • [26] Factors related to mortality of patients with COVID-19 who are admitted to the ICU: Prognostic mortality factors of COVID-19 patients
    alvarez-Macias, Alejandro
    ubeda-Iglesias, Alejandro
    Macias-Seda, Juana
    Gomez-Salgado, Juan
    MEDICINE, 2024, 103 (21) : E38266
  • [27] ASSOCIATION OF SERUM LIPID LEVELS WITH COVID-19 INFECTION, SEVERITY AND MORTALITY
    Chidambaram, V.
    Shanmugavel, H.
    Kumar, A.
    Voruganti, D.
    Mehta, J. L.
    JOURNAL OF INVESTIGATIVE MEDICINE, 2022, 70 (02) : 458 - 458
  • [28] Prognostic value of serum amyloid A in patients with COVID-19
    Li Cheng
    Jian-Zhong Yang
    Wen-Hui Bai
    Zhuan-Yun Li
    Li-Fang Sun
    Juan-Juan Yan
    Chen-Liang Zhou
    Bao-Peng Tang
    Infection, 2020, 48 : 715 - 722
  • [29] Prognostic value of serum amyloid A in patients with COVID-19
    Cheng, Li
    Yang, Jian-Zhong
    Bai, Wen-Hui
    Li, Zhuan-Yun
    Sun, Li-Fang
    Yan, Juan-Juan
    Zhou, Chen-Liang
    Tang, Bao-Peng
    INFECTION, 2020, 48 (05) : 715 - 722
  • [30] Association of the insulin resistance marker TyG index with the severity and mortality of COVID-19
    Huihui Ren
    Yan Yang
    Fen Wang
    Yongli Yan
    Xiaoli Shi
    Kun Dong
    Xuefeng Yu
    Shujun Zhang
    Cardiovascular Diabetology, 19