Application of high-throughput IEF-PAGE for EPO-doping testing

被引：8
|
作者：
Reichel, Christian [1 ]
机构：
[1] AIT Seibersdorf Labs, Doping Control Lab, A-2444 Seibersdorf, Austria
来源：
DRUG TESTING AND ANALYSIS | 2012年 / 4卷 / 09期
关键词：
erythropoietin; high-throughput screening; isoelectric focusing; IEF-PAGE; doping control; ISOELECTRIC PROFILES; ERYTHROPOIETIN; URINE; SAMPLES; SERUM;
D O I：
10.1002/dta.1411
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Since its first publication in 2000, the isoelectric focusing (IEF) method of erythropoietin (EPO) used in doping control has been considered a procedure with relatively small sample number capacity. To overcome this limitation, a variation of the current protocol was evaluated, which uses double-sized gels with 48120 wells plus three electrodes and hence multiplies the capacity of the electrophoretic chamber. With this modification up to 120 samples and standards can be run on a single gel thus, making IEF-PAGE of EPO a high-throughput method. The protocol is ideally suited for large-scale screening purposes. Copyright (c) 2012 John Wiley & Sons, Ltd.
引用
收藏
页码：728 / 732
页数：5
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