Overexpression of Rho-GDP-Dissociation Inhibitor-γ Inhibits Migration of Neural Stem Cells

被引：6

作者：

Wang, Jiao ^{[1
,2
]}

Li, Xiao ^{[1
]}

Cheng, Hua ^{[1
]}

Wang, Kai ^{[1
]}

Lu, Wei ^{[1
]}

Wen, Tieqiao ^{[1
,2
]}

机构：

[1] Shanghai Univ, Sch Life Sci, Inst Syst Biol, Lab Mol Neural Biol, Shanghai 200444, Peoples R China

[2] Shanghai Univ, Sch Commun & Informat Engn, Shanghai 200444, Peoples R China

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 2013年 / 91卷 / 11期

基金：

中国国家自然科学基金;

关键词：

neural stem cell; migration; Rac1; Rho-GDI; ACTIN CYTOSKELETON; GTPASES;

D O I：

10.1002/jnr.23261

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Neural stem cell (NSC) migration relies heavily on the regulation of actin and microtubule cytoskeletons by Rho GTPases, which are critical regulators of key steps during NSC migration. However, the migration mechanism remains unclear. Rho-GDP-dissociation inhibitor- (Rho-GDI) was identified as an important downregulator of the Rho family of GTPases, because of its ability to prevent nucleotide exchange and thus membrane association. This study investigates the role of Rho-GDI in neural stem cells migration. Our results indicate that the overexpression of Rho-GDI maintains NSCs in the stem cell state, meanwhile preventing NSC migration through inhibition of Rac1 expression, one of the Rho-family GTPases. This study provides the basis for further study of the molecular mechanism of NSC migration. (c) 2013 Wiley Periodicals, Inc.

引用

页码：1394 / 1401

页数：8

共 50 条

[31] Production of a monoclonal antibody against Rho-GDP dissociation inhibitor 1
Kim, Hyun-Suk
Jo, Eugene
Kim, Jae Won
Lee, Chang-Won
[J]. MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (10) : S2 - S2
[32] A Rho Kinase (ROCK) Inhibitor, Y-27632, Inhibits the Dissociation-Induced Cell Death of Salivary Gland Stem Cells
Kim, Kichul
Min, Sol
Kim, Daehwan
Kim, Hyewon
Roh, Sangho
[J]. MOLECULES, 2021, 26 (09):
[33] Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells
Keiichi Hatakeyama
Yorikane Fukuda
Keiichi Ohshima
Masanori Terashima
Ken Yamaguchi
Tohru Mochizuki
[J]. BMC Research Notes, 5 (1)
[34] Transgenic expression of Rho GDP dissociation inhibitor (Rho GDI), an endogenous inhibitor of Rho family of proteins, in mouse lens disrupts lens growth and structure
Maddala, R
Qiu, J
Pendurthi, B
Reneker, LW
Rao, PV
[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2005, 46
[35] Rho GDP-Dissociation Inhibitor 2 Inhibits C-X-C Chemokine Receptor Type 4-Mediated Acute Lymphoblastic Leukemia Cell Migration
Luo, Jixian
Wang, Junting
Zheng, Huiguang
Wang, Lan
[J]. FRONTIERS IN ONCOLOGY, 2020, 10
[36] Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor (Rho GDI) in Saccharomyces cerevisiae
Koch, G
Tanaka, K
Masuda, T
Yamochi, W
Nonaka, H
Takai, Y
[J]. ONCOGENE, 1997, 15 (04) : 417 - 422
[37] Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor (Rho GDI) in Saccharomyces cerevisiae
Gertrude Koch
Kazuma Tanaka
Tadayuki Masuda
Wataru Yamochi
Hidetaro Nonaka
Yoshimi Takai
[J]. Oncogene, 1997, 15 : 417 - 422
[38] Molecular and functional characterization of a Rho GDP dissociation inhibitor in the filamentous fungus Tuber borchii
Michele Menotta
Antonella Amicucci
Giorgio Basili
Emanuela Polidori
Vilberto Stocchi
Francisco Rivero
[J]. BMC Microbiology, 8
[39] Molecular and functional characterization of a Rho GDP dissociation inhibitor in the filamentous fungus Tuber borchii
Menotta, Michele
Amicucci, Antonella
Basili, Giorgio
Polidori, Emanuela
Stocchi, Vilberto
Rivero, Francisco
[J]. BMC MICROBIOLOGY, 2008, 8 (1)
[40] Loss of expression of LyGDI (ARHGDIB), a rho GDP-dissociation inhibitor, in Hodgkin lymphoma
Ma, Liya
Xu, Gaixiang
Sotnikova, Anna
Szczepanowski, Monika
Giefing, Maciej
Krause, Kristina
Krams, Matthias
Siebert, Reiner
Jin, Jie
Klapper, Wolfram
[J]. BRITISH JOURNAL OF HAEMATOLOGY, 2007, 139 (02) : 217 - 223

← 1 2 3 4 5 →