A facile one-step redox route for the synthesis of graphene/poly (3,4-ethylenedioxythiophene) nanocomposite and their applications in biosensing

In the present study, we for the first time propose a new one-step electrochemical redox route for the synthesis of high quality graphene-poly(3,4-ethylenedioxythiophene) (graphene-PEDOT) nanocomposite film based on simultaneous electrodeposition of PEDOT and electrochemical reduction of graphene oxide (GO) on a glassy carbon (GC) electrode. Data from scanning electron microscopy (SEM), UV-vis spectroscopy (UV-vis) and electrochemical impedance spectroscopy (EIS) demonstrated that the graphene/PEDOT nanocomposite film was successfully synthesized. The obtained graphene-PEDOT nanocomposite film showed large specific area, high conductivity, good biocompatibility, and fast redox properties and had encapsulated structures, which make it a novel material promising for biological applications. As an enzyme model, ascorbate oxidase (AO) was entrapped onto the film-modified electrode and used to construct an electrochemical ascorbic acid biosensor. The modified electrode showed good electrocatalytic performance towards ascorbic acid with high selectivity, wide linear range, and good stability. The facile and easy electrochemical approach used for the preparation of graphene-PEDOT/AO may open up new horizons in developing of cost effective biosensors. (C) 2013 Elsevier B.V. All rights reserved.