Altered expression of CCN1 in oral lichen planus associated with keratinocyte activation and IL-1β, ICAM1, and CCL5 up-regulation

被引：11

作者：

Wang, Yun ^{[1
]}

Du, Guanhuan ^{[2
]}

Shi, Linjun ^{[2
]}

Shen, Xuemin ^{[2
]}

Shen, Zhengyu ^{[1
]}

Liu, Wei ^{[3
,4
,5
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Dermatol, Sch Med, Shanghai, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Oral Med, Sch Med, Shanghai, Peoples R China

[3] Shanghai Jiao Tong Univ, Dept Oral & Maxillofacial Head & Neck Oncol, Shanghai Peoples Hosp 9,Coll Stomatol, Sch Med,Natl Clin Res Ctr Oral Dis,Shanghai Key L, Shanghai, Peoples R China

[4] Shanghai Jiao Tong Univ, Dept Oral & Maxillofacial Head & Neck Oncol, Shanghai Peoples Hosp 9,Coll Stomatol, Sch Med,Natl Clin Res Ctr Oral Dis,Shanghai Key L, Shanghai, Peoples R China

[5] Shanghai Res Inst Stomatol, Shanghai, Peoples R China

来源：

JOURNAL OF ORAL PATHOLOGY & MEDICINE | 2020年 / 49卷 / 09期

关键词：

CCL5; CCN1; ICAM1; IL-1; beta; keratinocyte; oral lichen planus; MALIGNANT-TRANSFORMATION; INFLAMMATION; P38;

D O I：

10.1111/jop.13087

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Background: Emerging evidence indicates that CCN1 is a novel inflammation-regulated mediator involved in the pathogenesis of some immune-mediated inflammatory diseases. The objective of this study was to investigate the preliminary roles of CCN1 and its related cytokines IL-1 beta, CCL5, and ICAM1 in oral lichen planus (OLP). Methods: CCN1 expression levels in biopsies from OLP patients against normal oral mucosa (NOM) using immunohistochemistry (42 OLP vs 9 NOM) and RT-qPCR (20 OLP vs 20 NOM) were compared, respectively. The correlation of CCN1 and IL-1 beta, CCL5, and ICAM1 expression was examined by RT-qPCR in tissue samples and an in vitro cell culture system using keratinocyte HaCaT cells incubated with lipopolysaccharides. Results: Immunohistochemistry showed that CCN1 protein mainly expressed in the cytoplasm of epithelial keratinocytes of OLP. Consistently, RT-qPCR revealed that mRNA expression of CCN1 was increased in OLP compared with NOM (P < .05) and positively correlated with the high expression of IL-1 beta, ICAM1, and CCL5 (P < .001), respectively. Importantly, an in vitro study showed that keratinocyte proliferation significantly (P < .05) increased by CCN1 stimulation. Moreover, IL-1 beta, ICAM1, and CCL5 expression in keratinocytes stimulated by CCN1 was increased (P < .05), respectively. Conclusions: This preliminary study for the first time reported that altered expression of CCN1 was associated with high expression of IL-1 beta, ICAM1, and CCL5 in OLP. And we demonstrated CCN1 promoted keratinocyte activation, as well as IL-1 beta, ICAM1, and CCL5 production in keratinocytes. Our data indicated that the potential role of CCN1 and its related cytokines was involved in the pathogenesis of OLP.

引用

页码：920 / 925

页数：6

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