Characterization of Listeria monocytogenes strains isolated from food and humans in Italy by pulsed-field gel electrophoresis

被引：7

作者：

Gianfranceschi, M ^{[1
]}

Pourshaban, M ^{[1
]}

Gattuso, A ^{[1
]}

Wedell-Neergaard, C ^{[1
]}

Aureli, R ^{[1
]}

机构：

[1] Ist Super Sanita, Food Lab, I-29900161 Rome, Italy

来源：

FOOD MICROBIOLOGY | 2002年 / 19卷 / 01期

关键词：

D O I：

10.1006/fmic.2001.0463

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pulsed-field gel electrophoresis (PFGE) was used to type 90 strains of Listeria monocytogenes isolated from clinical cases and from meat products in Italy in the period 1987-95. The objective of this retrospective study was to compare the genetic profiles to determine the existence of predominant clones and to evaluate their association with the sporadic cases of listeriosis reported in recent years in Italy. A total of 32 distinct PFGE types were identified: PFGE types 1 and 9 were identified both for strains isolated from clinical samples and those isolated from food The use of the PFGE molecular method in surveillance projects and epidemiological investigations could contribute to better understanding of microbial populations and could also be useful as part of the HACCP programme, in conducting controls along different points of the food production line. (C) 2002 Elsevier Science Ltd.

引用

页码：47 / 55

页数：9

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