DNA-FINGERPRINTING OF LISTERIA-MONOCYTOGENES STRAINS BY PULSED-FIELD GEL-ELECTROPHORESIS

被引：26
|
作者：
MOORE, MA [1 ]
DATTA, AR [1 ]
机构：
[1] US FDA,DIV MOLEC BIOL RES & EVALUAT,200 C ST SW,WASHINGTON,DC 20204
来源：
FOOD MICROBIOLOGY | 1994年 / 11卷 / 01期
关键词：
D O I：
10.1006/fmic.1994.1005
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Chromosomal DNA of Listeria monocytogenes strains was digested with SmaI restriction enzyme, and the resulting fragments were separated by pulsed-field gel electrophoresis. Distinctive banding patterns were obtained from the strains of major foodborne disease serotypes 1/2a, 1/2b and 4b. Matched sets of clinical and food isolates revealed close similarity among strains from a given episode and distinct differences among strains from separate episodes. © 1994 Academic Press Limited.
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页码：31 / 38
页数：8
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