Characterization and promoter activity of chromoplast specific carotenoid associated gene (CHRC) from Oncidium Gower!Ramsey

被引：23

作者：

Chiou, Chung-Yi ^{[1
]}

Wu, Keqiang ^{[1
]}

Yeh, Kai-Wun ^{[1
]}

机构：

[1] Natl Taiwan Univ, Coll Life Sci, Inst Plant Biol, Taipei 106, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 10期

关键词：

carotenoids; flower colour; orchid (Oncidium); plant pigments; tissue-specific promoter;

D O I：

10.1007/s10529-008-9767-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tissue-specific promoters are required for plant molecular breeding to drive a target gene in the appropriate location in plants. A chromoplast-specific, carotenoid-associated gene (OgCHRC) and its promoter (Pchrc) were isolated from Oncidium orchid and characterized. Northern blot analysis revealed that OgCHRC is specifically expressed in flowers, not in roots and leaves. Transient expression assay of Pchrc by bombardment transformation confirmed its differential expression pattern in floral tissues of different horticulture plants and cell-type location in conical papillate cells of adaxial epidermis of flower. These results suggest that Pchrc could serve as a useful tool in ornamental plant biotechnology to modify flower color.

引用

页码：1861 / 1866

页数：6

共 50 条

[31] Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity
Whyte, DA
Li, CY
Thomson, RB
Nix, SL
Zanjani, R
Karp, SL
Aronson, PS
Igarashi, P
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1999, 277 (04) : F587 - F598
[32] Characterization of promoter activity of the ethylene receptor gene OgERS1 from Oncidesa in transgenic Arabidopsis
Huang, W. -F.
Huang, P. -L.
Do, Y. -Y.
BIOLOGIA PLANTARUM, 2016, 60 (02) : 261 - 268
[33] CHARACTERIZATION OF THE HUMAN CD4 GENE PROMOTER - TRANSCRIPTION FROM THE CD4 GENE CORE PROMOTER IS TISSUE-SPECIFIC AND IS ACTIVATED BY ETS PROTEINS
SALMON, P
GIOVANE, A
WASYLYK, B
KLATZMANN, D
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (16) : 7739 - 7743
[34] Molecular cloning and functional characterization of a seed-specific VvβVPE gene promoter from Vitis vinifera
Gong, Peijie
Wei, Rong
Li, Yan
Wang, Ruipu
Tang, Yujin
Wang, Ling
Zhu, Huijun
Wang, Yuejin
Zhang, Chaohong
PLANTA, 2019, 250 (02) : 657 - 665
[35] Molecular cloning and functional characterization of a seed-specific VvβVPE gene promoter from Vitis vinifera
Peijie Gong
Rong Wei
Yan Li
Ruipu Wang
Yujin Tang
Ling Wang
Huijun Zhu
Yuejin Wang
Chaohong Zhang
Planta, 2019, 250 : 657 - 665
[36] IDENTIFICATION AND LOCALIZATION OF A DEVELOPMENTAL STAGE-SPECIFIC PROMOTER ACTIVITY FROM THE MURINE LAMBDA-5 GENE
YANG, J
GLOZAK, MA
BLOMBERG, BB
JOURNAL OF IMMUNOLOGY, 1995, 155 (05): : 2498 - 2514
[37] Characterization of FaRB7, a near root-specific gene from strawberry (Fragariaxananassa Duch.) and promoter activity analysis in homologous and heterologous hosts
Vaughan, Simon P.
James, David J.
Lindsey, Keith
Massiah, Andrea J.
JOURNAL OF EXPERIMENTAL BOTANY, 2006, 57 (14) : 3901 - 3910
[38] Isolation of 4-coumarate Co-A ligase gene promoter from loblolly pine (Pinus taeda) and characterization of tissue-specific activity in transgenic tobacco
Osakabe, Yuriko
Osakabe, Keishi
Chiang, Vincent L.
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2009, 47 (11-12) : 1031 - 1036
[39] Characterization of an anther- and tapetum-specific gene and its highly specific promoter isolated from tomato (vol 25, pg 231, 2006)
Chen, R. D.
Zimmermann, Elizabeth
Xu, S. X.
Liu, G. S.
Smith, Alan G.
PLANT CELL REPORTS, 2009, 28 (03) : 539 - 539
[40] Cellular localization of the embryo-specific Hybrid PRP from Zea mays, and characterization of promoter regulatory elements of its gene
Jose-Estanyol, M.
Puigdomenech, P.
PLANT MOLECULAR BIOLOGY, 2012, 80 (03) : 325 - 335

← 1 2 3 4 5 →