Optimization of multi-enzyme hydrolysis process for efficient corn gluten meal hydrolysis

In order to hydrolyze corn gluten meal efficiently, combinations of two or three out of three hydrolysis enzymes (protex6L, protex7L and papain) were used and the process parameters were optimized in this paper. The degree of hydrolysis of corn gluten meal was assayed by using pH-stat method. The ratio of enzymes was optimized and the effect of multi-enzyme hydrolysis was compared with that of single-enzyme hydrolysis. The result showed that the optimal ratio of protex6L and protex7L was 3:1, protex6L and papain was 6:5, protex7L and papain was 1:1, and the optimal ratio of protex6L, protex7L and papain was 4:1:1.The degree of hydrolysis and soluble protein content of multi-enzyme hydrolysis and single-enzyme hydrolysis were approximately equal, but multi-enzyme hydrolysis was timesaving.