Amphiphilic Polybetaines: The Effect of Side-Chain Hydrophobicity on Protein Adsorption

被引:56
|
作者
Colak, Semra [1 ]
Tew, Gregory N. [1 ]
机构
[1] Univ Massachusetts, Dept Polymer Sci & Engn, Conte Res Ctr Polymers, Amherst, MA 01003 USA
基金
美国国家科学基金会;
关键词
SURFACE-PROPERTIES; BLOCK-COPOLYMER; HYPERBRANCHED FLUOROPOLYMER; ANTIFOULING COATINGS; CONTACT-ANGLE; AIR-WATER; FIBRINOGEN; RESIST; IMMOBILIZATION; POLYACRYLATES;
D O I
10.1021/bm201791p
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Novel amphiphilic polybetaines were synthesized and used as the base material for nonfouling coatings. The amphiphilicity of these polybetaines was systematically tuned by coupling chains of increasing hydrophobicity to the zwitterionic functionality side at the repeat unit level. An oligoethylene glycol (OEG) moiety was selected to yield the most hydrophilic coating, while octyl (C-8) and fluorinated (F) groups were used to impart lipophilicity and lipophobicity to the coatings, respectively. This unique design allowed us to investigate the effect of the lipophilicity/lipophobicity of the side chain on the nonfouling properties of these zwitterionic systems. Adsorption studies, performed using six different proteins, showed that the fluorinated polybetaine, Poly[NFZI-co-NSi], resisted nonspecific adsorption as effectively as, and in some cases even better than, the most hydrophilic Poly[NOEGZI-co-NSi] coating. The comparison of Poly[NFZI-co-NSi] to its noncharged analog demonstrated the essential nature of the zwitterionic functionality in imparting nonfouling character to the coating.
引用
收藏
页码:1233 / 1239
页数:7
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