Use of the 2-Chlorotrityl Chloride Resin for Microwave-Assisted Solid Phase Peptide Synthesis

被引：24

作者：

Ieronymaki, Matthaia ^{[1
]}

Androutsou, Maria Eleni ^{[1
,2
]}

Pantelia, Anna ^{[1
]}

Friligou, Irene ^{[1
,2
]}

Crisp, Molly ^{[3
]}

High, Kirsty ^{[3
]}

Penkman, Kirsty ^{[3
]}

Gatos, Dimitrios ^{[1
]}

Tselios, Theodore ^{[1
]}

机构：

[1] Univ Patras, Dept Chem, Rion 26504, Greece

[2] Eldrug SA Pharmaceut Co, Platani 26504, Greece

[3] Univ York, Dept Chem, BioArCh, York YO10 5DD, N Yorkshire, England

来源：

BIOPOLYMERS | 2015年 / 104卷 / 05期

关键词：

solid phase peptide synthesis; microwave assisted peptide synthesis; 2-chlorotrityl chloride resin; racemization; MYELIN OLIGODENDROCYTE GLYCOPROTEIN; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; SCLEROSIS-LIKE DISEASE; MULTIPLE-SCLEROSIS; BASIC-PROTEIN; IMMUNODOMINANT EPITOPE; AMINO-ACIDS; INDUCTION; LIGANDS;

D O I：

10.1002/bip.22710

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast and efficient microwave (MW)-assisted solid-phase peptide synthesis protocol using the 2-chlorotrityl chloride resin and the Fmoc/tBu methodology, has been developed. The established protocol combines the advantages of MW irradiation and the acid labile 2-chlorotrityl chloride resin. The effect of temperature during the MW irradiation, the degree of resin substitution during the coupling of the first amino acids and the rate of racemization for each amino acid were evaluated. The suggested solid phase methodology is applicable for orthogonal peptide synthesis and for the synthesis of cyclic peptides. (C) 2015 Wiley Periodicals, Inc.

引用

页码：506 / 514

页数：9

共 50 条

[1] Use of the 2-Chlorotrityl Chloride Resin for Microwave-Assisted Solid Phase Peptide Synthesis
Department of Chemistry, University of Patras, Rion
26504, Greece
不详
26504, Greece
不详
Biopolymers, 5 (506-514):
[2] Microwave-assisted solid-phase peptide synthesis of the 60–110 domain of human pleiotrophin on 2-chlorotrityl resin
Irene Friligou
Evangelia Papadimitriou
Dimitrios Gatos
John Matsoukas
Theodore Tselios
Amino Acids, 2011, 40 : 1431 - 1440
[3] Microwave-assisted solid-phase peptide synthesis of the 60-110 domain of human pleiotrophin on 2-chlorotrityl resin
Friligou, Irene
Papadimitriou, Evangelia
Gatos, Dimitrios
Matsoukas, John
Tselios, Theodore
AMINO ACIDS, 2011, 40 (05) : 1431 - 1440
[4] A new method for the preparation of 2-chlorotrityl resin and its application to solid-phase peptide synthesis
Lee, Tae-Kyung
Ryoo, Sun-Jong
Lee, Yoon-Sik
TETRAHEDRON LETTERS, 2007, 48 (03) : 389 - 391
[5] APPLICATION OF 2-CHLOROTRITYL CHLORIDE IN CONVERGENT PEPTIDE-SYNTHESIS
ATHANASSOPOULOS, P
BARLOS, K
GATOS, D
HATZI, O
TZAVARA, C
TETRAHEDRON LETTERS, 1995, 36 (31) : 5645 - 5648
[6] Solid-phase synthesis via N-terminal attachment to the 2-chlorotrityl resin
Hoekstra, WJ
Greco, MN
Yabut, SC
Hulshizer, BL
Maryanoff, BE
TETRAHEDRON LETTERS, 1997, 38 (15) : 2629 - 2632
[7] Improving 2-Chlorotrityl Chloride (2-CTC) Resin Activation
Roman, Tanya
Acosta, Gerardo
de la Torre, Beatriz G.
Cardenas, Constanza
Guzman, Fanny
Albericio, Fernando
METHODS AND PROTOCOLS, 2023, 6 (05)
[8] 2-CHLOROTRITYL CHLORIDE RESIN - STUDIES ON ANCHORING OF FMOC-AMINO ACIDS AND PEPTIDE CLEAVAGE
BARLOS, K
CHATZI, O
GATOS, D
STAVROPOULOS, G
INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1991, 37 (06): : 513 - 520
[9] Rapid microwave-assisted solid phase peptide synthesis
Erdelyi, Mate
Gogoll, Adolf
PEPTIDE REVOLUTION: GENOMICS, PROTEOMICS & THERAPEUTICS, 2004, : 102 - 103
[10] Rapid microwave-assisted solid phase peptide synthesis
Erdélyi, M
Gogoll, A
BIOPOLYMERS, 2003, 71 (03) : 340 - 340

← 1 2 3 4 5 →