Effects of Doxorubicin, Cisplatin, and Tamoxifen on the Metabolic Profile of Human Breast Cancer MCF-7 Cells As Determined by 1H High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance

Doxorubicin (Doxo), cisplatin (Cis), and tamoxifen, (Tamo) are part of many chemotherapeutic regimens. However, there have been limited studies of the way metabolism in breast cancer is affected by chemotherapy. We studied, through H-1 high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy, the metabolic profile of human breast cancer MCF-7 control (Con) cells as well as MCF-7 cells treated with Tamo, Cis, and Doxo. H-1 HR-MAS NMR Single-pulse spectra evidenced signals from the cell compounds, including fatty acids (membranes), water-soluble proteins, and metabolites: The spectra showed that phosphocholine biomarker of breast cancer malignant transformation) signals were stronger in Con than in-treated cells. Betaine (i.e., the major osmolyte in cells) was Observed at similar concentrations in MCF-7 control and treated cells but was absent in nontumor MCF-10A cells. The NMR. spectra acquired with the Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence were used only in qualitative analyses-because the Signal areas were attenuated according to their transverse relaxation time (T-2). The CPMG method was used to identify soluble metabolites such as organic, acids, amino acids, Choline and its derivatives, taurine; and guanidine) acetate. H-1 HR-MAS NMR spectroscopy efficiently demonstrated the effects of Tame); Cis, and Doxo on the metabolic profile of MCF-7 cells. The fatty acid, phosphocholine, and choline variations observed by single-pulse HR-MAS NMR have the potential, to characterize both responder and nonresponder tumors at a molecular level.