Evaluation of a Caprine Arthritis-Encephalitis Virus/Maedi-Visna Virus Indirect Enzyme-Linked Immunosorbent Assay in the Serological Diagnosis of Ovine Progressive Pneumonia Virus in US Sheep

被引：17
|
作者：
Herrmann-Hoesing, Lynn M. [1 ,2 ]
Broughton-Neiswanger, Liam E. [2 ]
Gouine, Kimberly C. [2 ]
White, Stephen N. [1 ,2 ,3 ]
Mousel, Michele R. [4 ]
Lewis, Gregory S. [4 ]
Marshall, Katherine L. [5 ]
Knowles, Donald P. [1 ,2 ]
机构：
[1] Washington State Univ, USDA, ARS, Anim Dis Res Unit, Pullman, WA 99164 USA
[2] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA
[3] Washington State Univ, Ctr Integrated Biotechnol, Pullman, WA 99164 USA
[4] ARS, USDA, US Sheep Expt Stn, Dubois, ID 83423 USA
[5] Ctr Epidemiol & Anim Hlth, Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO 80526 USA
来源：
CLINICAL AND VACCINE IMMUNOLOGY | 2010年 / 17卷 / 02期
关键词：
SMALL RUMINANT LENTIVIRUSES; PHYLOGENETIC ANALYSIS; HETEROGENEITY; ANTIBODIES;
D O I：
10.1128/CVI.00349-09
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
A caprine arthritis-encephalitis virus (CAEV)/maedi-visna virus (MVV) indirect enzyme-linked immunosorbent assay (iELISA) was validated with samples from U. S. sheep and by the use of radioimmunoprecipitation as the standard for comparison. The sensitivity and the specificity were 86.0% (+/- 5.8%) and 95.9% (+/- 2.9%), respectively. The iELISA format and phylogenetic differences based on the MVV gag sequence contribute to the reduced sensitivity.
引用
收藏
页码：307 / 310
页数：4
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