Expression and purification of the Bacillus subtilis thioredoxin superfamily protein YkvV

被引：3
|
作者：
Tanaka, R
Araki, Y
Mizukami, M
Miyauchi, A
Ishibashi, M
Tokunaga, H
Tokunaga, M
机构：
[1] Kagoshima Univ, Fac Agr, Kagoshima 8900065, Japan
[2] Higeta Shoyu Co, Dept Res & Dev, Chiba 2888680, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2004年 / 68卷 / 08期
关键词：
Brevibacillus choshinensis; Bacillus subtilis; ykvV; thiol-disulfide oxidoreductase; secretion;
D O I：
10.1271/bbb.68.1801
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bacillus subtilis YkvV protein, an extracellular thioredoxin superfamily protein, was successfully expressed both in Brevibacillus choshinensis culture medium using an efficient promoter and the secretion signal of its surface layer protein, and in Escherichia coli cytoplasm with the amino-terminal His-tag (His-YkvV). His-YkvV was purified to homogeneity by Ni-NTA column. Both secreted YkvV and purified His-YkvV exhibited thioldisulfide oxidoreductase activity.
引用
收藏
页码：1801 / 1804
页数：4
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