N-3 PUFA Prevent Oxidative Stress in a Rat Model of Beta-Amyloid-Induced Toxicity

被引:16
|
作者
Morgese, Maria Grazia [1 ]
Schiavone, Stefania [1 ]
Bove, Maria [1 ]
Colia, Anna Laura [1 ]
Dimonte, Stefania [1 ]
Tucci, Paolo [1 ]
Trabace, Luigia [1 ]
机构
[1] Univ Foggia, Dept Clin & Expt Med, I-71122 Foggia, Italy
关键词
n-3; PUFA; oxidative stress; beta-amyloid; inflammation;
D O I
10.3390/ph14040339
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Polyunsaturated fatty acids (PUFA) are involved in brain disorders associated to amyloid beta (A beta) toxicity for which oxidative stress, neurochemical dysfunctions, and neuroinflammation are underlying mechanisms. Here, mechanisms through which lifelong exposure to n-3 PUFA-enriched or n-6/n-3 balanced diets could elicit a protective role in a rat model of A beta-induced toxicity were investigated. To this aim, we quantified hippocampal reactive oxygen species (ROS) amount, 8-hydroxy-2 '-deoxyguanosine and interleukin-10 levels, NADPH oxidase (NOX) 1, NOX2, superoxide dismutase 1, and glutathione contents, as well as plasmatic malondialdehyde. Moreover, in the same experimental groups, we assessed tryptophan, serotonin, and its turnover, kynurenine, and noradrenaline amounts. Results showed increased hippocampal ROS and NOX2 levels, serotonin turnover, kynurenine, and noradrenaline contents in A beta-treated rats. Both n-6/n-3 balanced and n-3 PUFA enriched diets reduced ROS production, NOX1 and malondialdehyde levels, serotonin turnover, and kynurenine amount in A beta-injected rats, while increasing NOX2, superoxide dismutase 1, and serotonin contents. No differences in plasmatic coenzyme Q10, reduced glutathione (GSH) and tryptophan levels were detected among different experimental groups, whereas GSH + oxidized glutathione (GSSG) levels were increased in sham animals fed with n-3 PUFA enriched diet and in A beta-treated rats exposed to both n-6/n-3 balanced and n-3 enriched diets. In addition, A beta-induced decrease of interleukin-10 levels was prevented by n-6/n-3 PUFA balanced diet. N-3 PUFA enriched diet further increased interleukin-10 and 8-hydroxy-2 '-deoxyguanosine levels. In conclusion, our data highlight the possible neuroprotective role of n-3 PUFA in perturbation of oxidative equilibrium induced by A beta-administration.
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页数:17
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