Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Aspergillus niger

被引：51
|
作者：
Aifa, MS [1 ]
Sayadi, S [1 ]
Gargouri, A [1 ]
机构：
[1] Ctr Biotechnol Sfax, Sfax, Tunisia
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 10期
关键词：
Aspergillus niger; heterologous expression; lignin peroxidase; Phanerochaete chrysosporium;
D O I：
10.1023/A:1005541206270
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The lignin peroxidase (isoenzyme H8) of Phanerochaete chrysosporium was expressed in Aspergillus niger under the control of plant nopaline synthase (NOS) promoter and terminator. H8 mRNA was produced in this heterologous system. Western blot analysis showed that the recombinant protein was of size similar to the native H8. The extracellular lignin peroxidase activity in these primary constructs was positive, though weak (1.12 nKat mg(-1)).
引用
收藏
页码：849 / 853
页数：5
相关论文
共 50 条
  • [41] Hyperactivation and thermostabilization of Phanerochaete chrysosporium lignin peroxidase by immobilization in xerogels
    Asgher, Muhammad
    Asad, M. Javaid
    Bhatti, Haq Nawaz
    Legge, Raymond L.
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2007, 23 (04): : 525 - 531
  • [42] MOLECULAR ANALYSIS OF A PHANEROCHAETE-CHRYSOSPORIUM LIGNIN PEROXIDASE GENE
    WALTHER, I
    KALIN, M
    REISER, J
    SUTER, F
    FRITSCHE, B
    SALOHEIMO, M
    LEISOLA, M
    TEERI, T
    KNOWLES, JKC
    FIECHTER, A
    GENE, 1988, 70 (01) : 127 - 137
  • [43] Reactive oxygen species and induction of lignin peroxidase in Phanerochaete chrysosporium
    Belinky, PA
    Flikshtein, N
    Lechenko, S
    Gepstein, S
    Dosoretz, CG
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (11) : 6500 - 6506
  • [44] HETEROLOGOUS EXPRESSION OF ACTIVE MANGANESE PEROXIDASE FROM PHANEROCHAETE-CHRYSOSPORIUM USING THE BACULOVIRUS EXPRESSION SYSTEM
    PEASE, EA
    AUST, SD
    TIEN, M
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 179 (02) : 897 - 903
  • [45] LIGNIN PEROXIDASE AND MANGANESE PEROXIDASE - EXTRACELLULAR LIGNINASES FROM PHANEROCHAETE-CHRYSOSPORIUM
    GOLD, MH
    WARIISHI, H
    RENGANATHAN, V
    VALLI, K
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1988, 196 : 388 - INOR
  • [46] INFLUENCE OF LIGNIN PEROXIDASE CONCENTRATION AND LOCALIZATION IN LIGNIN BIODEGRADATION BY PHANEROCHAETE-CHRYSOSPORIUM
    KUREK, B
    ODIER, E
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1990, 34 (02) : 264 - 269
  • [47] Influence of the physical state of lignin on its degradability by the lignin peroxidase of Phanerochaete chrysosporium
    Kurek, Bernard
    Monties, Bernard
    Odier, Etienne
    Integration, the VLSI Journal, 1990, 9 (03) : 771 - 777
  • [48] Removal of hazardous compounds by lignin peroxidase from Phanerochaete chrysosporium
    Manimekalai, R
    Swaminathan, T
    BIOPROCESS ENGINEERING, 2000, 22 (01) : 29 - 33
  • [49] EFFECTS OF TEMPERATURE ON THE PRODUCTION OF MANGANESE PEROXIDASE AND LIGNIN PEROXIDASE BY PHANEROCHAETE-CHRYSOSPORIUM
    VYAS, BRM
    VOLC, J
    SASEK, V
    FOLIA MICROBIOLOGICA, 1994, 39 (01) : 19 - 22
  • [50] Isolation and heterologous expression of the Phanerochaete chrysosporium calmodulin gene
    Sakamoto, Takaiku
    Honda, Yoichi
    Kameshita, Isamu
    Suzuki, Kazumi
    Irie, Toshikazu
    MYCOSCIENCE, 2013, 54 (03) : 241 - 246
12345