ECHS1 acts as a novel HBsAg-binding protein enhancing apoptosis through the mitochondrial pathway in HepG2 cells

被引：30
|
作者：
Xiao, Chuan-Xing [1 ]
Yang, Xiao-Ning [1 ]
Huang, Qing-Wen [1 ]
Zhang, Yu-Qin [1 ,2 ,3 ]
Lin, Bi-Yun [1 ]
Liu, Jing-Jing [1 ]
Liu, Yun-Peng [1 ]
Jazag, Arnarsanaa [3 ]
Guleng, Bayasi [1 ,2 ]
Ren, Jian-Lin [1 ]
机构：
[1] Xiamen Univ, Zhongshan Hosp, Dept Gastroenterol, Xiamen 361004, Fujian Province, Peoples R China
[2] Xiamen Univ, Fac Clin Med, Coll Med, Xiamen 361005, Fujian Province, Peoples R China
[3] 3rd Gen Hosp, Natl Inst Med Res, Ulaanbaatar, Mongolia
来源：
CANCER LETTERS | 2013年 / 330卷 / 01期
基金：
中国国家自然科学基金;
关键词：
ECHS1; HBs binding protein; HepG2 cell apoptosis; HEPATITIS-B-VIRUS; HEPATOCELLULAR-CARCINOMA; ENOYL-COENZYME; PROTEOMIC ANALYSIS; GENE-EXPRESSION; SHORT-CHAIN; LIVER; HEPATOCYTES; IDENTIFICATION; REPLICATION;
D O I：
10.1016/j.canlet.2012.11.030
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We aimed to confirm the role of ECHS1 as a binding protein of HBsAg (HBs) and investigate its function during the development of hepatocellular carcinoma (HCC). Our results show that both exogenous and endogenous ECHS1 proteins bind to HBs and co-localize in the cytoplasm in vitro. The coexistence of HBs and ECHS1 enhances HepG2 cell apoptosis, affects ECHS1 localization in the mitochondria and induces apoptosis by decreasing the mitochondrial membrane potential (MMP). These findings suggest that ECHS1 may be applied as a potential therapeutic target during the treatment of HBV-related hepatitis or HCC. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：67 / 73
页数：7
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