Electrochemical immunosensors for detection of microorganisms

被引：0

作者：

Skladal, Petr ^{[1
,2
]}

Kovar, David ^{[1
,2
]}

Krajicek, Vit ^{[1
]}

Siskova, Petra ^{[1
,3
,4
]}

Pribyl, Jan ^{[2
]}

Svabenska, Eva ^{[2
]}

机构：

[1] Masaryk Univ, Fac Sci, Dept Biochem, CS-61137 Brno, Czech Republic

[2] Masaryk Univ, CEITEC MU, Brno 60500, Czech Republic

[3] Masaryk Univ, Fac Med, Inst Microbiol, Brno, Czech Republic

[4] St Annes Univ Hosp, Brno, Czech Republic

来源：

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2013年 / 8卷 / 02期

关键词：

Amperometric Sensor; Screen-Printed Electrode; Immunochemical Detector; Atomic Force Microscopy; BIOLOGICAL WARFARE AGENTS; ESCHERICHIA-COLI; RAPID DETECTION; SALMONELLA-TYPHIMURIUM; BIOSENSOR; BACTERIA; IDENTIFICATION; ASSAYS;

D O I：

暂无

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Electrochemical immunosensing devices for detection of microbial agents are briefly reviewed. The practical results describe amperometric immunosensors based on screen-printed electrodes as a general platform for sandwich-based assay of microbes. The examples are focused on the determination of Francisella, Salmonella, Escherichia and Bacillus species. The achieved analytical parameters seem promising for real applications. In particular, combination of the immunosensor with cyclone device allowed fully automated testing of the system for detection of bioaerosols of E. coli as a model agent; the preliminary results confirm that levels below 100 CFU/L in air can be detected within 20 min.

引用

页码：1635 / 1649

页数：15

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