Fecal microbiota transplantation from young donor mice improves ovarian function in aged mice

被引:19
|
作者
Xu, Li [1 ]
Zhang, Qiankun [2 ,3 ]
Dou, Xiaowei [1 ,4 ]
Wang, Yipeng [2 ]
Wang, Jianwei [2 ,5 ]
Zhou, Yong [1 ,6 ]
Liu, Xingyin [1 ,2 ]
Li, Jing [1 ]
机构
[1] Nanjing Med Univ, State Key Lab Reprod Med, Nanjing 211166, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Dept Pathogen Biol, Microbiol Div, State Key Lab Reprod Med, Nanjing 211166, Jiangsu, Peoples R China
[3] Shanghai Personal Biotechnol, Shanghai 200231, Peoples R China
[4] Nanjing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Nanjing 210003, Jiangsu, Peoples R China
[5] Soochow Univ, Childrens Hosp, Inst Pediat Res, Suzhou 215003, Jiangsu, Peoples R China
[6] Guangdong Second Prov Gen Hosp, Reprod Med Ctr, Fertil Preservat Lab, Guangzhou 510317, Guangdong, Peoples R China
关键词
Ovarian aging; Gut microbiota; Inflammatory cytokines; Ovarian microenvironment; LIFE-SPAN; GUT MICROBIOTA; INFLAMMATION; MACROPHAGES;
D O I
10.1016/j.jgg.2022.05.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Advanced maternal age is characterized by declines in the quantity and quality of oocytes in the ovaries, and the aging process is accompanied by changes in gut microbiota composition. However, little is known about the relationship between gut microbiota and ovarian aging. By using fecal microbiota transplantation (FMT) to transplant material from young (5-week-old) into aged (42-week-old) mice, we find that the composition of gut microbiota in FMT-treated mice presents a "younger-like phenotype" and an increase of commensal bacteria, such as Bifidobacterium and Ruminococcaceae. Moreover, the FMT-treated mice show increased anti-inflammatory cytokine IL-4 and decreased pro-inflammatory cytokine IFN-g. Fertility tests for assessing ovarian function reveal that the first litter size of female FMT-treated mice is significantly higher than that of the non-FMT group. Morphology analysis demonstrates a dramatic decrease in follicle atresia and apoptosis as well as an increase in cellular proliferation in the ovaries of the FMT-treated mice. Our results also show that FMT improves the immune microenvironment in aged ovaries, with decreased macrophages and macrophage-derived multinucleated giant cells (MNGCs). These results suggest that FMT from young donors could be a good choice for delaying ovarian aging.Copyright (c) 2022, The Authors. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码:1042 / 1052
页数:11
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