Versatile two-phase solvent system for anthraquinone prefractionation by high speed countercurrent chromatography

被引：9
|
作者：
Yua, LM [1 ]
Chen, XX [1 ]
Ai, P [1 ]
Zi, M [1 ]
Wu, P [1 ]
Li, ZY [1 ]
机构：
[1] Yunnan Normal Univ, Dept Chem, Kunming 650092, Peoples R China
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2001年 / 24卷 / 19期
关键词：
D O I：
10.1081/JLC-100107349
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In order to find a high-speed countercurrent chromatography solvent system that can be used as a general prefractionation step for anthraquinone, the crude extracts of five traditional Chinese medicines, Radix rubiae, Rhizoma polygoni cuspidati, Semen cassiae, Radix et rhizoma rhei, and Aloe were separated with a two-phase system composed of CHCl3:CH3OH:H2O = 4:x:2 in which x was between 3 and 4 for different samples. The fractionated components were identified on TLC. This solvent system gave a good separation in most of the anthraquinones.
引用
收藏
页码：2961 / 2970
页数：10
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