Investigation of clonal relationships of K. pneumoniae isolates from neonatal intensive care units by PFGE and rep-PCR

被引：8

作者：

Koroglu, Mehmet ^{[1
]}

Ozbek, Ahmet ^{[1
]}

Demiray, Tayfur ^{[1
]}

Hafizoglu, Taner ^{[2
]}

Guclu, Ertugrul ^{[3
]}

Altindis, Mustafa ^{[1
]}

Karabay, Oguz ^{[3
]}

Durmaz, Riza ^{[4
,5
]}

机构：

[1] Sakarya Univ, Dept Med Microbiol, Sch Med, TR-54290 Adapazari, Sakarya, Turkey

[2] Sakarya Univ, Dept Pediat, Sch Med, TR-54290 Adapazari, Sakarya, Turkey

[3] Sakarya Univ, Dept Infect Dis, Sch Med, TR-54290 Adapazari, Sakarya, Turkey

[4] Yildirim Beyazit Univ, Fac Med, Dept Clin Microbiol, Ankara, Turkey

[5] Publ Hlth Agcy Turkey, Dept Microbiol, Mol Microbiol Reseach & Applicat Lab, Reference Labs,Minist Hlth, Ankara, Turkey

来源：

JOURNAL OF INFECTION IN DEVELOPING COUNTRIES | 2015年 / 9卷 / 08期

关键词：

K; pneumoniae; clonal relationship; pulsed-field gel electrophoresis; repetitive PCR; epidemic; neonatal intensive care unit; RESISTANT KLEBSIELLA-PNEUMONIAE; FIELD GEL-ELECTROPHORESIS; SEQUENCE-BASED PCR; ESCHERICHIA-COLI; TYPING METHODS; SYSTEM; COLONIZATION; INFECTIONS; OUTBREAK; EPIDEMIOLOGY;

D O I：

10.3855/jidc.6326

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Introduction: Clonal relationships of Klebsiella pneumoniae strains obtained during an epidemic and after a one-year post-epidemic (non-epidemic) period in the same neonatal intensive care unit (NICU) using pulsed-field gel electrophoresis (PFGE) and repetitive polymerase chain reaction (rep-PCR) by the DiversiLab (DL) system were investigated, and the results of both molecular techniques were evaluated. Methodology: Fifteen K. pneumoniae strains were included in this study. All identified bacterial strains were confirmed by 16S rDNA sequencing and analyzed by PFGE and the DL system. Results: According to the PFGE results, 15 isolates showed 10 different band profiles. Nine of these 15 isolates were included in one of the formed clusters, and the remaining six isolates were not included in any of them. According to the DL system results, 15 isolates showed two different clusters, with three strains in one cluster and four strains in the other. The remaining strains could not be placed any one of the clusters. PFGE was used as the gold standard based on its strong genetic discriminatory power. The DL system results showed that PFGE missed the relationship of the two epidemic-related strains and demonstrated one epidemic-unrelated strain to be epidemic related. Conclusions: Both systems may easily be used for clonal relationships of K. pneumoniae strains. The DL system was clearly more rapid and convenient than PFGE, but its discriminatory power seemed to be inferior to that of PFGE based on 15 K. pneumoniae strains.

引用

页码：829 / 836

页数：8