New cell lines from larval fat bodies of Spodoptera exigua:: Characterization and susceptibility to baculoviruses (Lepidoptera: Noctuidae)

被引:28
|
作者
Zhang, H
Zhang, YA [1 ]
Qin, QL
Li, X
Miao, L
Wang, YZ
Yang, ZQ
Ding, C
机构
[1] Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China
[2] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100080, Peoples R China
基金
中国国家自然科学基金;
关键词
Spodoptera exigua; fat body; cell line; karyotype; DAF-PCR; nuclear polyhedrosis viruses;
D O I
10.1016/j.jip.2005.10.004
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Two new cell lines, designated IOZCAS-Spex-II and IOZCAS-Spex-III, were initiated from the fat bodies of larvae of the beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae) in TNM-FH medium containing 10% fetal bovine serum. The spherical cells were predominant among the various cell types and measures approximately 15 pin in diameter. The cell lines were mainly composed of tetraploid cells with chromosome numbers ranging from 116 to 131 (n = 31). The cell lines were confirnied to have originated from the S. exigua by DAF-PCR technique. They were susceptible to the multiple nucleocapsid nuclear polyhedrosis viruses from S. exigua. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:9 / 12
页数:4
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