Piggery wastewater was fractionated using three types of separation technologies including particle size fractionation, resin adsorption, and polar diversity separation. In polar diversity fractions, alkanes were the main non-polar components while phenol-like compounds such as phthalates acid esters (PAEs), and other non-polar esters accounted for more than 50% of the aromatic components. Sterols and fatty acid methyl esters (FAMEs) accounted for more than 70% of the non-hydrocarbon components. Six hazardous pharmaceuticals, including dihydroxanthin, a-santonin, bulflomedil, zolpidem, flumazenil, and pimozide, were identified in the non-hydrocarbons fraction of the piggery wastewater.