Three phase partitioning for simultaneous extraction of oil, protein and polysaccharide from rice bran

被引:20
|
作者
Wang, Hong [1 ,2 ]
Geng, Haoyuan [1 ]
Chen, Jun [1 ]
Wang, Xu [1 ]
Li, Dan [1 ]
Wang, Tong [1 ]
Yu, Dianyu [1 ]
Wang, Liqi [3 ]
机构
[1] Northeast Agr Univ, Sch Food Sci, Harbin 150030, Peoples R China
[2] Jilin Business & Technol Coll, Sch Grain, Changchun 130507, Peoples R China
[3] Harbin Univ Commerce, Sch Comp & Informat Engn, Harbin 150028, Peoples R China
关键词
Three phase partitioning; Rice bran; Extraction; Response surface methodology; MICROWAVE-ASSISTED EXTRACTION; PURIFICATION; OPTIMIZATION; SEPARATION; RECOVERY; LIPIDS;
D O I
10.1016/j.ifset.2020.102447
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Three phase partitioning (TPP) technique was used to extract oil, protein and polysaccharides simultaneously from rice bran. The fatty acid composition of rice bran oil (RBO) and structure of rice bran protein (RBP) and rice bran polysaccharides (RBPS) were analyzed. Under the optimal conditions of (NH4)(2)SO4 concentration 28% (w/v), slurry to t-butanol ratio 1:1.1 (v/v), pH 5.10, extraction temperature 40 degrees C and extraction time 1 h, the highest extraction yields of RBO, RBP and RBPS were 17.28%, 6.81% and 2.09%, respectively. The fatty acid composition of RBO was analyzed by GC, the structure of RBP was analyzed by FTIR and SDS-PAGE, and the structure of RBPS was analyzed by FTIR. The results were similar to those obtained by other methods. Therefore, TPP can be used as an effective technology for simultaneous extraction of oil, protein and polysaccharide from rice bran.
引用
收藏
页数:10
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