The Pseudomonas type III effector HopQ1 activates cytokinin signaling and interferes with plant innate immunity

被引:87
|
作者
Hann, Dagmar R. [1 ,2 ]
Dominguez-Ferreras, Ana [1 ,2 ]
Motyka, Vaclav [3 ]
Dobrev, Petre I. [3 ]
Schornack, Sebastian [4 ]
Jehle, Anna [5 ]
Felix, Georg [5 ]
Chinchilla, Delphine [1 ,2 ]
Rathjen, John P. [6 ]
Boller, Thomas [1 ,2 ]
机构
[1] Univ Munich LMU, Inst Genet, D-82152 Martinsried, Germany
[2] Univ Basel, Inst Bot, Sect Plant Physiol, CH-4056 Basel, Switzerland
[3] Inst Expt Bot AS CR, Prague 16502 6, Lysolaje, Czech Republic
[4] Univ Cambridge, Sainsbury Lab, Cambridge CB2 1LR, England
[5] Univ Tubingen, ZMBP Zentrum Mol Biol Pflanzen, Forschungsgrp Pflanzenbiochem, D-72076 Tubingen, Germany
[6] Australian Natl Univ, Canberra, ACT 0200, Australia
基金
新加坡国家研究基金会;
关键词
cytokinin; flagellin; FLS2; HopQ1; innate immunity; type III effector; NUCLEOSIDE HYDROLASE; BACTERIAL VIRULENCE; RECEPTOR FLS2; EARLY EVENTS; ARABIDOPSIS; SYRINGAE; KINASE; PHOSPHORYLATION; RESISTANCE; EXPRESSION;
D O I
10.1111/nph.12544
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We characterized the molecular function of the Pseudomonas syringae pv. tomato DC3000 (Pto) effector HopQ1.In silico studies suggest that HopQ1 might possess nucleoside hydrolase activity based on the presence of a characteristic aspartate motif. Transgenic Arabidopsis lines expressing HopQ1 or HopQ1 aspartate mutant variants were characterized with respect to flagellin triggered immunity, phenotype and changes in phytohormone content by high-performance liquid chromatography-MS (HPLC-MS). We found that HopQ1, but not its aspartate mutants, suppressed all tested immunity marker assays. Suppression of immunity was the result of a lack of the flagellin receptor FLS2, whose gene expression was abolished by HopQ1 in a promoter-dependent manner. Furthermore, HopQ1 induced cytokinin signaling in Arabidopsis and the elevation in cytokinin signaling appears to be responsible for the attenuation of FLS2 expression. We conclude that HopQ1 can activate cytokinin signaling and that moderate activation of cytokinin signaling leads to suppression of FLS2 accumulation and thus defense signaling.
引用
收藏
页码:585 / 598
页数:14
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