CRYOPRESERVATION OF MOUSE GAMETES AND EMBRYOS

被引:2
|
作者
Landel, Carlisle P. [1 ,2 ]
机构
[1] Thomas Jefferson Univ, Dept Microbiol & Immunol, Philadelphia, PA 19107 USA
[2] Thomas Jefferson Univ, Kimmel Canc Ctr, Philadelphia, PA 19107 USA
关键词
IN-VITRO FERTILIZATION; TRANSGENIC MICE; SPERM; VITRIFICATION; SURVIVAL; SPERMATOZOA; FERTILITY; FROZEN;
D O I
10.1016/S0076-6879(10)76006-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
As the use of genetically engineered mouse models continues to expand, the need to cryopreserve strains of mice increases in parallel in order to preserve these unique research resources and provide a low-cost alternative to maintaining the large inventory of strains. This chapter discusses methods for the cryopreservation of mouse embryos, sperm, and oocytes, and briefly discusses other requirements for implementing a successful cryopreservation program.
引用
收藏
页码:85 / 105
页数:21
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