Polarization-resolved second harmonic generation microscopy with a four-channel Stokes-polarimeter

被引：45

作者：

Mazumder, Nirmal ^{[1
]}

Qiu, Jianjun ^{[1
]}

Foreman, Matthew R. ^{[2
]}

Romero, Carlos Macias ^{[2
]}

Hu, Chih-Wei ^{[1
]}

Tsai, Han-Ruei ^{[1
]}

ToeroeK, Peter ^{[2
]}

Kao, Fu-Jen ^{[1
]}

机构：

[1] Natl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan

[2] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Blackett Lab, London SW7 2BZ, England

来源：

OPTICS EXPRESS | 2012年 / 20卷 / 13期

关键词：

EMISSION MICROSCOPY; IMAGING MICROSCOPY; MUELLER MATRICES; COLLAGEN; TISSUES; LIGHT; DECOMPOSITION; FLUORESCENCE; DIAGNOSTICS; VECTORS;

D O I：

10.1364/OE.20.014090

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We developed a four-channel photon counting based Stokes-polarimeter for spatial characterization of polarization effects in second harmonic generation (SHG). We have implemented a calibration technique allowing quantitative measurement of polarization parameters, such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy from the acquired Stokes parameters. The technique is used as contrast mechanism to characterize the polarization properties from two potassium dihydrogen phosphate (KDP) micro-crystals and collagen type-I in SHG microscopy. (C) 2012 Optical Society of America

引用

页码：14090 / 14099

页数：10

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