Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

被引：1
|
作者：
Mazumder, Nirmal [1 ]
Qiu, Jianjun [1 ]
Hu, Chih-Wei [1 ]
Kao, Fu-Jen [1 ]
机构：
[1] Natl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan
来源：
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIII | 2013年 / 8588卷
关键词：
Nonlinear microscopy; Polarimetry; Ultrafast optics; Instrumentation; measurement; and metrology; POLARIZATION; COLLAGEN;
D O I：
10.1117/12.2003894
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.
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页数：7
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