Do House Wrens (Troglodytes aedon) add spider egg cases to their nests for heterospecific cleaning?

House Wrens (Troglodytes aedon Vieillot, 1809) regularly add spider egg cases (Arachnida: Araneae) to their nests, which may be an example of heterospecific cleaning. This behavior involves one animal employing another to remove parasites from their nests. In House Wren nests, juvenile spiders hatching from egg cases may facilitate the reduction of mites that feed on nestlings. We tested this ectoparasite reduction hypothesis by monitoring House Wren nests for spider egg cases and by collecting completed nests to compare the number of spider egg cases and Dermanyssus hirundinis (Hermann, 1804) mites. No significant relationship was found between the number of spider egg cases and number of D. hirundinis mites in nests. We also found no significant relationship between the number of D. hirundinis mites in nests and the body condition of nestlings. Finally, no significant difference was found between the number of D. hirundinis mites in early versus late season nests, but significantly more spider egg cases were added to late season nests. Of a subsample of spider egg cases dissected, we found that 28% contained spider eggs or embryos. We also identified three species of juvenile spiders from House Wren nests using DNA barcoding. Overall, we found no evidence that spider egg cases reduce the number of D. hirundinis mites or engender better quality offspring in House Wren nests.