Template-Directed Preparation of Bovine Serum Albumin Microporous Multilayer Films

The fabrication of polymeric materials with ordered submicron-size void structures is potentially valuable for many applications such as catalysts, separation and adsorbent media. This paper reports the preparation of macroporous protein multilayer films with regular voids using silica nanospheres as templates. Both monodisperse silica colloids and highly ordered assembly silica multilayer films are used as templates to prepare microporous bovine serum albumin multilayer films with ruleless and ordered submicron-sized voids. Glutaraldehyde is used as a crosslinking agent to form a firm net-like protein film on the surface of silica templates. The microporous protein film is obtained after removing of silica templates. Compare with polymer film, protein film has good biocompatibility and biodegradability which will be beneficial to its biological applications.