Transformation of the Oomycete Saprolegnia monoica to hygromycin-B resistance

被引：12
|
作者：
MortBontemps, M
Fevre, M
机构：
[1] Lab. de Biologie Cellulaire Fongique, CGMC UMR CNRS 5534, Université Lyon 1, F-69622 Villeurbanne Cedex, 43
来源：
CURRENT GENETICS | 1997年 / 31卷 / 03期
关键词：
transformation; Oomycete; Saprolegnia monoica; hygromycin;
D O I：
10.1007/s002940050205
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Saprolegnia monoica was stably transformed to hygromycin resistance using the plasmids pTH210 and pHAM34H containing a bacterial phosphotransferase gene fused to regulatory sequences from genes of another Oomycete. Vectors pBT6, pCM54, used to transform higher fungi, yielded no stable transformants. DNA hybridizations indicated that transformation resulted from a single-copy integration of the transforming vector. Development of non-resistant subcultures from transformed colonies revealed that the transgene could become silent and was not uniformly expressed in the transformants.
引用
收藏
页码：272 / 275
页数：4
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